(Get Answer)

Be sure to use the correct units and indicate them clearly at all stages. Please show all work. If you used an Excel spreadsheet, please attach. There is a receptor kinase called LRK2 involved in sensing lactose in the bacterium Lactobacillus smolenski. When it binds lactose, it catalyzes the following reaction: RR4 stands for Response Regulator 4, a small protein that becomes phosphorylated on a specific Asp residue, forming a mixed acid anhydride (RR4-Pi). The phosphorylated RR4 can then bind and regulate other proteins in the signal transduction cascade. In order to obtain insight into the mechanism of this kinase, you perform a kinetic analysis with varying amounts of ATP and RR4. Each reaction contains 10 pM of the LRK2 kinase. You do the assay in the presence of a saturating concentration of lactose, which keeps the enzyme maximally active. (This will not affect the analysis. A) With which of these mechanisms are most consistent with these data for LRK2? Briefly justify your conclusion. "Ping-Pong" mechanism Ternary complex with ordered pathway (which one binds first?) Ternary complex with random pathway B) Determine the following parameters for the LRK2 receptor kinase: Km and/or Kd for ATP Km and/or Kd for RR4 kcat of the enzyme